Synedica NAD+ & B12 Kit

What NAD+ and B12 Are

Nicotinamide adenine dinucleotide (NAD+) is a pyridine nucleotide that functions as a redox cofactor, carrying electrons between reactions in glycolysis, the citric acid cycle and oxidative phosphorylation. It also acts as a substrate — not just a cofactor — for NAD+-consuming enzymes including sirtuins, poly(ADP-ribose) polymerases (PARPs) and CD38, which link it to DNA repair, chromatin regulation and cellular stress signalling. It carries CAS number 53-84-9, molecular formula C21H27N7O14P2, and a molecular weight of approximately 663.4 g/mol.

Vitamin B12 is a cobalt-containing corrinoid vitamin studied as a cofactor for two enzymes: methionine synthase, which converts homocysteine to methionine, and methylmalonyl-CoA mutase, which converts methylmalonyl-CoA to succinyl-CoA. These reactions are central to one-carbon metabolism, methylation and DNA synthesis research. The synthetic, research-grade form most commonly supplied is cyanocobalamin, CAS number 68-19-9, molecular formula C63H88CoN14O14P, molecular weight approximately 1,355.4 g/mol. NAD+ and B12 are chemically unrelated molecules — a nucleotide and a corrinoid vitamin respectively — studied together because both feed into overlapping metabolic and methylation pathways relevant to cellular energy research.

What Current Research Says

A 2024 review in Nature Reviews Molecular Cell Biology by Migaud, Ziegler and Baur describes NAD+ as a cofactor or substrate in an estimated one-quarter of all cellular biochemical reactions, noting that mitochondrial NAD+ concentrations (around 250 μM) substantially exceed nuclear and cytosolic pools (around 100 μM), and that the gut microbiome contributes to NAD+ precursor availability by converting nicotinamide to nicotinic acid (Migaud, Ziegler & Baur, 2024, Nat Rev Mol Cell Biol). The same review states that long-term human safety data across different NAD+-precursor supplementation strategies remain an open research question.

A pharmacokinetic pilot study published in Frontiers in Aging Neuroscience tracked plasma and urine NAD+ metabolites during a 6-hour intravenous NAD+ infusion in human participants. It found NAD+ was "rapidly and completely removed from the plasma for at least the first 2 h" of infusion, with plasma NAD+ levels reaching roughly 400% above baseline only by the 6-hour mark, and identified NAD+ glycohydrolase and NAD+ pyrophosphatase activity as likely degradation pathways, with no adverse events recorded during the procedure (Grant et al., 2019, Front Aging Neurosci).

For B12, a 2023 systematic review and meta-analysis in Frontiers in Endocrinology pooled data from 66 studies and nearly 88,000 patients and reported that higher vitamin B12 levels were inversely associated with metabolic syndrome (odds ratio 0.87), while higher homocysteine levels were positively associated with it (odds ratio 1.19) (Ulloque-Badaracco et al., 2023, Front Endocrinol). The review's authors note that the biological mechanisms behind these population-level associations require further investigation. None of these findings constitute a treatment, prevention or wellness claim for this research-use product; they describe what has been studied in the published literature on the two molecules, not what a laboratory-use compound sold by Synedica UK does for an individual.

Specifications

Purity, Batch Testing and Certificate of Analysis Standards

Every Synedica NAD+ & B12 Kit offered for sale is supplied with third-party analytical documentation rather than a single purity percentage repeated across listings. A Certificate of Analysis (COA) should show the specific batch or lot number, the analytical method used (typically HPLC), the measured purity result for that batch, and the issuing laboratory. Because NAD+ is a redox-sensitive molecule that can degrade with heat, light and repeated freeze-thaw cycling, batch age and storage history are as relevant to research validity as the initial purity figure. Request batch-specific documentation before relying on any listing's headline purity claim — see our contact page to request a Certificate of Analysis for a specific batch.

Sourcing and Verification

Buying NAD+ and B12 from a UK-based supplier does not remove the need to verify what arrives. Before ordering, check that the listing states research-use status clearly, that batch documentation is offered rather than promised only "on request," and that the compound identity (CAS number, molecular formula) is independently checkable against a source such as PubChem. The Synedica NAD+ & B12 Kit is offered for sale on this basis, with documentation available per batch. Researchers working across the wider NAD+ pathway may also find the Synedica NAD+ & NMN Kit relevant, and the full catalogue is browsable on the research products page.